Disodium inosinate is generally produced from meat or from fish.

In the food industry, inosinic acid and its salts such as disodium inosinate are used as flavour enhancers.

It is often used in synergy with disodium inosinate; the combination is known as disodium 5'-ribonucleotides.

Disodium inosinate is used as a flavor enhancer, in synergy with monosodium glutamate (MSG) to provide the umami taste.

The food additives disodium inosinate and disodium guanylate, which are ribonucleotides, are usually used in synergy with monosodium glutamate-containing ingredients.

Disodium inosinate (E631) is the disodium salt of inosinic acid with the chemical formula CHNNaOP.

Inosinate can be manufactured into various compounds with the aid of genetically modified organisms: disodium inosinate E 631, dipotassium inosinate E 632, and dicalcium inosinate E 633.